#161
Trafficking Pathways in Root Tip

Stephanie Robert (P.I.: Natasha Raikhel and Zhenbiao Yang)

Jae-Ung Hwang and Shundai Li

It is known that there are genetic links between auxin transport and the endomembrane system. The screen was to examine directly the localization of PIN2::PIN2-GFP by Pathway HT automated imaging workstation. It is a fully integrated system for performing high throughput confocal imaging experiments on slides, dishes or multiwell plates. SCREEN DETAILS: For the screen a total of 2016 diverse chemicals were screened. The library was the Selected chemicals from Microsource Spectrum (SP). It was screened at a concentration of 50-100uM in 400ul of medium using our custom chemical 48-well plates. Seeds were germinated on media with chemicals, and PIN2-GFP localization was observed after 5 days. Overall, chemical compounds that altered marker localization or/and cell shape at least for two repetition, were selected. SUMMARY STATISTICS: Of the 2016 chemicals screened, a total of 163 (7.9%) were selected after the first observation (primary screen). After a repetition, 49 chemicals (2.4%) were confirmed in altered PIN2-GFP localization/expression.

NSF: 2010-0520325

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In total, 49 compounds from 1 library are annotated. Out of these, 3 have a score of 1 or higher, and 0 are annotated as inactive.

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Screen-level Files

Screen strategy

#15324
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Compound Files

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Microsource Spectrum 00100068
LibraryMicrosource Spectrum
ID00100068
NamePRIEURIANIN
FormulaC38H50O16
Molecular Weight 762.794
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irregular cell shape

#163
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Microsource Spectrum 01504017
LibraryMicrosource Spectrum
ID01504017
NameSAPINDOSIDE-A
FormulaC41H66O12
Molecular Weight 750.956
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Assay 1: 2

#8809
Assay 1: visual phenotype
  • Description None
  • Score 2: active in primary and secondary screen
  • Concentration 50-100 uM (25 ug/ml) in 0.5% DMSO

irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#164
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Microsource Spectrum 01504123
LibraryMicrosource Spectrum
ID01504123
Name10-HYDROXYCAMTOTHECIN
FormulaC20H16N2O5
Molecular Weight 364.351
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#165
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Microsource Spectrum 01500864
LibraryMicrosource Spectrum
ID01500864
NameHARMALINE
FormulaC13H14N2O
Molecular Weight 214.263
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irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#166
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Microsource Spectrum 01504059
LibraryMicrosource Spectrum
ID01504059
NameCHRYSANTHELLIN-A
FormulaC58H94O25
Molecular Weight 1191.352
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irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#167
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Microsource Spectrum 01500177
LibraryMicrosource Spectrum
ID01500177
NameCHLORHEXIDINE
FormulaC22H32Cl4N10
Molecular Weight 578.369
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#168
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Microsource Spectrum 01500362
LibraryMicrosource Spectrum
ID01500362
NameKETOCONAZOLE
FormulaC26H28Cl2N4O4
Molecular Weight 531.431
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irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#169
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Microsource Spectrum 01501156
LibraryMicrosource Spectrum
ID01501156
NameSULFAMETHOXYPYRIDAZINE
FormulaC11H12N4O3S
Molecular Weight 280.303
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#170
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Microsource Spectrum 01501155
LibraryMicrosource Spectrum
ID01501155
NameSULFAMETER
FormulaC11H12N4O3S
Molecular Weight 280.303
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#171
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Microsource Spectrum 01501147
LibraryMicrosource Spectrum
ID01501147
NameSULFAMONOMETHOXINE
FormulaC11H12N4O3S
Molecular Weight 280.303
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#172
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Microsource Spectrum 01502130
LibraryMicrosource Spectrum
ID01502130
NameAMINOCYCLOPROPANECARBOXYLIC-ACID
FormulaC4H7NO2
Molecular Weight 101.104
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#173
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Microsource Spectrum 01503906
LibraryMicrosource Spectrum
ID01503906
NameANISOMYCIN
FormulaC14H19NO4
Molecular Weight 265.305
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#174
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Microsource Spectrum 01500538
LibraryMicrosource Spectrum
ID01500538
NameSPECTINOMYCIN-HYDROCHLORIDE
FormulaC14H26Cl2N2O7
Molecular Weight 405.271
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#175
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Microsource Spectrum 01500551
LibraryMicrosource Spectrum
ID01500551
NameSULFAPYRIDINE
FormulaC11H11N3O2S
Molecular Weight 249.289
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#176
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Microsource Spectrum 01500549
LibraryMicrosource Spectrum
ID01500549
NameSULFAMETHIZOLE
FormulaC9H10N4O2S2
Molecular Weight 270.331
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#177
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Microsource Spectrum 01500547
LibraryMicrosource Spectrum
ID01500547
NameSULFAMERAZINE
FormulaC11H12N4O2S
Molecular Weight 264.304
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#178
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Microsource Spectrum 01500546
LibraryMicrosource Spectrum
ID01500546
NameSULFADIAZINE
FormulaC10H10N4O2S
Molecular Weight 250.277
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#179
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Microsource Spectrum 01503253
LibraryMicrosource Spectrum
ID01503253
NameMETHYLBENZETHONIUM-CHLORIDE
FormulaC28H44ClNO2
Molecular Weight 462.108
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reduced PIN2-GFP signal

50-100 um chemical (left) compared to DMSO control (right). Results: reduced PIN2-GFP

#180
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Microsource Spectrum 01503806
LibraryMicrosource Spectrum
ID01503806
NameHOMIDIUM-BROMIDE
FormulaC21H20BrN3
Molecular Weight 394.308
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changed localization or expression of PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: mislocalization or abnormal expression of PIN2-GFP

#181
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Microsource Spectrum 00330032
LibraryMicrosource Spectrum
ID00330032
NameDICAMBA
FormulaC8H6Cl2O3
Molecular Weight 221.037
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short root with normal PIN2-GFP localization

50-100 um chemical (left) compared to DMSO control (right). Results: short root with normal PIN2-GFP localization

#182
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Microsource Spectrum 01503610
LibraryMicrosource Spectrum
ID01503610
NameBENZALKONIUM-CHLORIDE
FormulaC22H40ClN
Molecular Weight 354.013
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#183
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Microsource Spectrum 00330040
LibraryMicrosource Spectrum
ID00330040
Name2,4-DICHLOROPHENOXYACETIC-ACID
FormulaC8H6Cl2O3
Molecular Weight 221.037
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#184
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Microsource Spectrum 00330041
LibraryMicrosource Spectrum
ID00330041
Name2,4-DICHLOROPHENOXYBUTYRIC-ACID
FormulaC10H10Cl2O3
Molecular Weight 249.091
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#185
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Microsource Spectrum 01501143
LibraryMicrosource Spectrum
ID01501143
NameSULFAPHENAZOLE
FormulaC15H14N4O2S
Molecular Weight 314.362
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#186
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Microsource Spectrum 00330044
LibraryMicrosource Spectrum
ID00330044
Name2,4-DICHLOROPHENOXYACETIC-ACID,-METHYL-ESTER
FormulaC9H8Cl2O3
Molecular Weight 235.064
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#187
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