#161

Trafficking Pathways in Root Tip

It is known that there are genetic links between auxin transport and the endomembrane system. The screen was to examine directly the localization of PIN2::PIN2-GFP by Pathway HT automated imaging workstation. It is a fully integrated system for performing high throughput confocal imaging experiments on slides, dishes or multiwell plates. SCREEN DETAILS: For the screen a total of 2016 diverse chemicals were screened. The library was the Selected chemicals from Microsource Spectrum (SP). It was screened at a concentration of 50-100uM in 400ul of medium using our custom chemical 48-well plates. Seeds were germinated on media with chemicals, and PIN2-GFP localization was observed after 5 days. Overall, chemical compounds that altered marker localization or/and cell shape at least for two repetition, were selected. SUMMARY STATISTICS: Of the 2016 chemicals screened, a total of 163 (7.9%) were selected after the first observation (primary screen). After a repetition, 49 chemicals (2.4%) were confirmed in altered PIN2-GFP localization/expression.

NSF: 2010-0520325

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In total, 1 compound from 1 library is annotated. Out of these, 3 have a score of 1 or higher, and 0 are annotated as inactive.