#161
Trafficking Pathways in Root Tip

Stephanie Robert (P.I.: Natasha Raikhel and Zhenbiao Yang)

Jae-Ung Hwang and Shundai Li

It is known that there are genetic links between auxin transport and the endomembrane system. The screen was to examine directly the localization of PIN2::PIN2-GFP by Pathway HT automated imaging workstation. It is a fully integrated system for performing high throughput confocal imaging experiments on slides, dishes or multiwell plates. SCREEN DETAILS: For the screen a total of 2016 diverse chemicals were screened. The library was the Selected chemicals from Microsource Spectrum (SP). It was screened at a concentration of 50-100uM in 400ul of medium using our custom chemical 48-well plates. Seeds were germinated on media with chemicals, and PIN2-GFP localization was observed after 5 days. Overall, chemical compounds that altered marker localization or/and cell shape at least for two repetition, were selected. SUMMARY STATISTICS: Of the 2016 chemicals screened, a total of 163 (7.9%) were selected after the first observation (primary screen). After a repetition, 49 chemicals (2.4%) were confirmed in altered PIN2-GFP localization/expression.

NSF: 2010-0520325

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In total, 49 compounds from 1 library are annotated. Out of these, 3 have a score of 1 or higher, and 0 are annotated as inactive.

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Screen-level Files

Screen strategy

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Compound Files

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Microsource Spectrum 00330049
LibraryMicrosource Spectrum
ID00330049
Name2,4,5-TRICHLOROPHENOXYACETIC-ACID,-ISOOCTYL-ESTER
FormulaC16H21Cl3O3
Molecular Weight 367.695
Use in structure search

Assay 1: 2

#8788
Assay 1: visual phenotype
  • Description None
  • Score 2: active in primary and secondary screen
  • Concentration 50-100 uM (25 ug/ml) in 0.5% DMSO

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#188
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Microsource Spectrum 01504153
LibraryMicrosource Spectrum
ID01504153
NameNORCANTHARIDIN
FormulaC8H8O4
Molecular Weight 168.147
Use in structure search

reduced PM PIN2-GFP signal

50-100 um chemical (left) compared to DMSO control (right). Results: Reduced PIN2-GFP

#189
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Microsource Spectrum 01500811
LibraryMicrosource Spectrum
ID01500811
NameBERBERINE-CHLORIDE
FormulaC20H18ClNO4
Molecular Weight 371.814
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#190
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Microsource Spectrum 01500520
LibraryMicrosource Spectrum
ID01500520
NamePYRIMETHAMINE
FormulaC12H13ClN4
Molecular Weight 248.711
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#191
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Microsource Spectrum 01500169
LibraryMicrosource Spectrum
ID01500169
NameCETYLPYRIDINIUM-CHLORIDE
FormulaC21H38ClN
Molecular Weight 339.986
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#192
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Microsource Spectrum 01504252
LibraryMicrosource Spectrum
ID01504252
NameCYTOCHALASIN-A
FormulaC29H35NO5
Molecular Weight 477.592
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irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#193
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Microsource Spectrum 01504121
LibraryMicrosource Spectrum
ID01504121
NameCYTOCHALASIN-B
FormulaC29H37NO5
Molecular Weight 479.608
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irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#194
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Microsource Spectrum 01500186
LibraryMicrosource Spectrum
ID01500186
NameCHLORTETRACYCLINE-HYDROCHLORIDE
FormulaC22H24Cl2N2O8
Molecular Weight 515.341
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#195
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Microsource Spectrum 01505252
LibraryMicrosource Spectrum
ID01505252
NamePALMATINE
FormulaC21H22NO5
Molecular Weight 368.403
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#196
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Microsource Spectrum 01502114
LibraryMicrosource Spectrum
ID01502114
Namep-FLUOROPHENYLALANINE
FormulaC9H10FNO2
Molecular Weight 183.180
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#197
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Microsource Spectrum 01502112
LibraryMicrosource Spectrum
ID01502112
NameCYCLOHEXIMIDE
FormulaC15H23NO4
Molecular Weight 281.347
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cytosolic and reduced PiIN2-GFP signal a

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic and reduced PIN2-GFP

#198
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Microsource Spectrum 00410019
LibraryMicrosource Spectrum
ID00410019
NameCYTOCHALASIN-D
FormulaC30H37NO6
Molecular Weight 507.618
Use in structure search

irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#199
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Microsource Spectrum 01505800
LibraryMicrosource Spectrum
ID01505800
NameCYTOCHALASIN-E
FormulaC28H33NO7
Molecular Weight 495.564
Use in structure search

irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#200
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Microsource Spectrum 01500814
LibraryMicrosource Spectrum
ID01500814
NameCANTHARIDIN
FormulaC10H12O4
Molecular Weight 196.200
Use in structure search

reduced PM PIN2-GFP signal

50-100 um chemical (left) compared to DMSO control (right). Results: Reduced PIN2-GFP

#201
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Microsource Spectrum 01500375
LibraryMicrosource Spectrum
ID01500375
NameMECHLORETHAMINE
FormulaC5H11Cl2N
Molecular Weight 156.054
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#202
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Microsource Spectrum 01500674
LibraryMicrosource Spectrum
ID01500674
NameMYCOPHENOLIC-ACID
FormulaC17H20O6
Molecular Weight 320.337
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cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#203
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Microsource Spectrum 01503226
LibraryMicrosource Spectrum
ID01503226
NameMECLOFENOXATE-HYDROCHLORIDE
FormulaC12H17Cl2NO3
Molecular Weight 294.174
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#204
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Reference
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Microsource Spectrum 01503223
LibraryMicrosource Spectrum
ID01503223
NamePARAROSANILINE-PAMOATE
FormulaC42H33N3O6
Molecular Weight 675.728
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#205
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Reference
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Microsource Spectrum 00100242
LibraryMicrosource Spectrum
ID00100242
Name14-METHOXY-4,4-BISNOR-8,11,13-PODOCARPATRIEN-3-ONE
FormulaC16H20O2
Molecular Weight 244.329
Use in structure search

irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#206
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Microsource Spectrum 01503094
LibraryMicrosource Spectrum
ID01503094
NameTHIOXOLONE
FormulaC7H4O3S
Molecular Weight 168.170
Use in structure search

Assay 1: 2

#8767
Assay 1: visual phenotype
  • Description None
  • Score 2: active in primary and secondary screen
  • Concentration 50-100 uM (25 ug/ml) in 0.5% DMSO

irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: irregular cell shape

#207
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Microsource Spectrum 01500673
LibraryMicrosource Spectrum
ID01500673
NameMOLSIDOMINE
FormulaC9H14N4O4
Molecular Weight 242.232
Use in structure search

cytosolic PIN2-GFP and irregular cell shape

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic amd irregular cell shape

#208
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Microsource Spectrum 01503059
LibraryMicrosource Spectrum
ID01503059
NameFLOXURIDINE
FormulaC9H11FN2O5
Molecular Weight 246.192
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#209
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Microsource Spectrum 01500550
LibraryMicrosource Spectrum
ID01500550
NameSULFAMETHOXAZOLE
FormulaC10H11N3O3S
Molecular Weight 253.278
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#210
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Microsource Spectrum 01500222
LibraryMicrosource Spectrum
ID01500222
NameDAPSONE
FormulaC12H12N2O2S
Molecular Weight 248.301
Use in structure search

cytosolic PIN2-GFP

50-100 um chemical (left) compared to DMSO control (right). Results: cytosolic localization

#211
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